My research investigates how using biochar to increase soil carbon content affects nitrogen transformation processes in vineyard soil from South East Queensland. Biochar is organic matter e.g. woodchip that is cooked at very high temperatures without oxygen producing a carbon dense product. Adding carbon to the soil is a Regenerative Agricultural principle that sequestrates carbon, improves soil and plant health and increases water and nutrient retention as well as microbial activity and crop output. Soil microbes are largely responsible for transforming atmospheric nitrogen into a form that is easily assessable for plants to take up in their roots for growth. My study focuses on how different rates of biochar under different moisture regimes affect the nitrogen cycling processes of soil microbes in local viticulture soil.